Extended Data Fig. 2: SEPT6-GFP localization and the effects of septin inhibition.
From: Blebs promote cell survival by assembling oncogenic signalling hubs
(A) Mouse SEPT6-GFP probe localization in MV3 cells embedded in soft bovine collagen. Maximum intensity projections (left) and single z-slices of 0.16 micron thickness (right) shown for representative cells in two columns. (B) Maximum intensity projections of mouse SEPT6-GFP probe localization in MV3 cells with and without inhibition by FCF or SEPT2(33–306) expression. Cells embedded in soft bovine collagen. (Below) Fraction of cell surface comprised of blebs for MV3 cells with and without SEPT6-GFP expression, FCF treatment, and SEPT2(33–306) expression. Dashed lines separate quartiles. Dots represent individual cells. (C) Mouse SEPT6-GFP probe localization in MV3 cells embedded in soft bovine collagen. Maximum intensity projections and single z-slices of 0.16 micron thickness shown for representative cells in two columns. (Above) Cells expressing the SEPT2(33–306) mutant. (Below) Cells expressing cytosolic mCherry (not shown) using the same pLVX-IRES-Hyg vector as the SEPT2(33–306) construct. Included as a negative control demonstrating that the septin mislocalization in SEPT2(33–306)-expressing cells is not due to effects arising from the protein expression construct.
