Extended Data Fig. 4: A mutation that disrupts curvature sensing blocks septin localization to the cell surface.
From: Blebs promote cell survival by assembling oncogenic signalling hubs
Top, cartoon illustrating the mechanism underlying the altered function of the SEPT6(ΔAH)-GFP mutant. Bottom left, mean local SEPT6(ΔAH)-GFP intensity and intracellular mean curvature as a function of distance from bleb edges, calculated from 630 blebs across 5 MV3 cells. Error bands indicate 95% confidence intervals. Bottom right, difference in mean local septin intensity compared to local maxima as a function of distance from bleb edge for both SEPT6-GFP (as seen in Fig. 2e) and SEPT6(ΔAH)-GFP.
