Extended Data Fig. 4: Vascular density in lung normal tissues and tumor tissues measured by microCT and endothelial marker.
From: Spatial mapping of mitochondrial networks and bioenergetics in lung cancer
a,b, Reconstruction of vascular structure (a) segmented from microCT images (b) by gaussian and binary morphological filters in OXPHOSLO LUSC (left panel) and OXPHOSHI LUAD (right panel) tumors. The density of vasculature was indicated. c,d, Representative of immunohistochemical staining of CD 34 on the section of a OXPHOSLO LUSC tumor (c, left panel). The threshold of positive CD 34 staining was identified using QuPath and indicated by red labeling (c, right panel). Scale bar (c) = 500 μm. Selected normal tissue region and tumor region in black boxes were zoomed-in (d). Scale bar (d) = 50 μm. e,f, Density of positive CD 34 staining in normal tissues (n = 10) and tumor tissues (n = 10) (e), and in LUAD (n = 6) and LUSC (n = 6) (f). Data are mean ± s.e.m., unpaired two-tailed t-test.
