Extended Data Fig. 7: Cryo-EM analysis of FKS1 S643P, a drug-resistant mutant of the enzyme.
From: Structural and mechanistic insights into fungal β-1,3-glucan synthase FKS1
a, A representative cryo-EM micrograph of FKS1 S643P from 9623 collected micrographs. b, Representative 2D class averages. c, Flow chart of cryo-EM data acquisition and data processing of FKS1 S643P. See Methods for more details. d, The gold-standard Fourier shell correlation (FSC) curve of the reconstructed map. e, The FSC curve between model and map, which is calculated by PHENIX.mtriage58. f, Cutaway views of the angular distribution of all particles used in the final 3D reconstruction. g, Local resolution distribution of the final cryo-EM map of FKS1 S643P, calculated with ResMap. h, Fit of cryo-EM map with FKS1 S643P model in example regions.
