Extended Data Fig. 2: Enzymatic hydrolysis and glycosyl linkage (methylation) analysis of FKS1-synthesized products.
From: Structural and mechanistic insights into fungal β-1,3-glucan synthase FKS1
a, Quantification of the released reducing sugars during enzymatic digestion of the water-insoluble polymer synthesized by GDN-purified FKS1 S643P. Two specific glucanases were used: specific endo-1,3-β-glucanase and endo-1,4-β-glucanase. Data are mean ± SEM, n = 3 independent experiments. b–c, The hydrolysis specificity of endo-1,3-β-Glucanase and endo-1,4-β-Glucanase was tested against standard polysaccharide β-1,3-glucan curdlan (b) and cellulose (c). A mixture of glucose (G1), laminaribiose (G2), laminaritriose (G3) and laminarihexaose (G6) was used as the standards (lane M). d, Mass spectrum of GC peak (11.64min) in (Fig. 1f) reveals the identity of this PMAA as 1,3,5-tri-O-acetyl-2,4,6-tri-O-methyl glucitol, confirming 1,3-Glcp linkage.
