Extended Data Fig. 3: Cryo-EM structural determination of S. cerevisiae FKS1 and structural features of FKS1 cytoplasmic region.
From: Structural and mechanistic insights into fungal β-1,3-glucan synthase FKS1
a, A representative cryo-EM micrograph of FKS1 from 11606 collected micrographs. b, Representative 2D class averages. c, Flow chart of cryo-EM data acquisition and data processing of FKS1. See Methods for more details. d, The gold-standard Fourier shell correlation (FSC) curve of the reconstructed map. e, The FSC curve between model and map, which is calculated by PHENIX.mtriage58. f, Cutaway views of the angular distribution of all particles used in the final 3D reconstruction. g, Local resolution distribution of the final cryo-EM map of FKS1, calculated with ResMap54. h, Topological diagram of FKS1 GT-___domain. Flexible segments invisible in the 3D reconstruction are depicted by dashed lines. i, Interactions between AC and GT ___domain. Labeled are central β strands and the structural elements involved in the extensive contacting interface: α2-5, α8-11, loops Lα9-α10 and Lα11-α12 of AC ___domain; α31, α34-35, loops Lβ5-β6, Lβ7-β8; Lβ8-β9 of GT ___domain). The FKS1 R319, a previously identified essential residue for FKS124, is marked by its Cα atom as sphere and is embedded in this interface. j, Zoomed in view of the boxed region in (i), showing the interaction between R319 and N1087 to maintain the interface between AC and GT ___domain.
