Extended Data Fig. 12: Genetic interactions among Hox and Gli3 genes.
From: The little skate genome and the evolutionary emergence of wing-like fins
a. ChIP-seq experiment in mouse embryonic branchial arches performed in Amin et al. 2015, which shows the binding profile of HoxA2 to Gli3 genomic locus. b. Whole-mount in situ hybridization of gli3 in zebrafish embryos inyected with a hoxd13a-GR mRNA. Developing fins are indicated with red arrowheads. In the absence of dexamethasone (left panel), the construct is inactive and the embryos develop normally (50 out of 57, 88%). Upon treatment with dexamethasone (right panel), hoxd13a is activated and causes a reduction of gli3 expression at the developing fin region (mild reduction in 39 out of 93, 42%; strong reduction in 22 out of 93, 24%). Scale bars = 250 µm.
