Extended Data Fig. 6: SSM binding interface footprinting results were consistent with the design model and crystal structure.
From: De novo design of modular peptide-binding proteins by superhelical matching
a, Using a PPL repeat-peptide binder as an example, a heat map presenting enrichment analysis for each mutation is generated. In each cell, the red colour indicates enrichment, and the blue colour indicates depletion. Wild-type sequences are indicated in the cells labelled with amino-acid one-letter codes. The mutants missing in the expression library are labelled with asterisks. Two positions (109Q and 156Q) are highlighted as examples showing conserved positions. Almost all mutations other than the wild type in these two positions are greatly depleted. b, Illustration shows the SSM region (orange), and the two conserved positions (109Q and 156Q in yellow).
