Extended Data Fig. 3: Change in multicellular neighbourhoods across the intestine.

A) Immune neighbourhood percentages as a percent of immune neighbourhoods. B) Neighbourhood percentages for Microvasculature and CD8+ T cell IEL neighbourhoods compared for donors with or without a history of hypertension (Microvasculature p value = 0.0065<, CD8+ T Enriched IEL p value = 0.0017 by two-sided T test, n = 3-5 donors). C) Difference in composition in neighbourhood by cell type for all neighbourhoods based on subtracting the log2 fold enrichment of each cell type found within that neighbourhood compared to average percentages in the tissue in SB from CL. Neighbourhoods and cell types are ordered by summing the absolute value of all rows and columns to denote conservation of a neighbourhood. D) 22 unique intestinal multicellular neighbourhoods (y axis of heatmap) were defined by enriched cell types (x axis of heatmap) as compared to overall percentage of cell types in the samples with 2 unique neighbourhoods not identified with overall neighbourhood analysis. E) Epithelial neighbourhood percentages as a percent of epithelial neighbourhoods from multicellular analysis performed on each individual region of the intestine separately. F) Immune neighbourhood percentages as a percent of immune neighbourhoods from multicellular analysis performed on each individual region of the intestine separately. (* p value< 0.05, ** p value< 0.01, *** p value < 0.001 by two-sided T test, n = 8 donors).