Fig. 1: Cryo-EM structure of the TnpB–ωRNA–target DNA ternary complex.
From: Cryo-EM structure of the transposon-associated TnpB enzyme
a, Schematic of the D. radiodurans ISDra2 locus. The MGE consists of the tnpA and tnpB genes flanked by the left end (LE) and right end (RE) elements of the transposon. The ωRNA is derived from the 3′ end of the tnpB gene and the RE element. b, Diagram of the ωRNA and target DNA used for cryo-EM analysis. The target strand (TS) and non-target strand (NTS) each comprise 35 nucleotides, and the non-target strand contains a TTGAT TAM sequence. The 247-nt ωRNA was co-expressed and co-purified with TnpB. Nucleotides −231 to −117, −70 to −49, −20 to −17 and 13 to 16 of the ωRNA, nucleotides −8 to 4 and 27 of the target strand, and nucleotides −11* and 1* to 24* of the non-target strand were not included in the final model. c, The ___domain structure of TnpB. CTD, C-terminal ___domain. Residues 281 to 296 and 379 to 408 were not included in the final model. d, Cryo-EM density map of the TnpB–ωRNA–target DNA complex. e, The overall structure of the TnpB–ωRNA–target DNA complex. Disordered regions are indicated as dotted lines.
