Extended Data Fig. 7: ωRNA architecture and recognition.
From: Cryo-EM structure of the transposon-associated TnpB enzyme
(a) Structural comparison of the ωRNA scaffold of TnpB with the guide RNA scaffolds of Cas12f (PDB ID: 7C7L) and Cas12a (PDB ID: 6I1K). Cas12f associates with its cognate RNA scaffold formed by crRNA and tracrRNA, whereas Cas12a uses only crRNA. Although these RNAs lack sequence similarity, they contain conserved PK structures. PK, pseudoknot; R:AR, repeat-antirepeat. (b) Recognitions of the PK architectures by TnpB, Cas12f, and Cas12a. The PK architectures are recognized by their cognate proteins in similar manners. (c) Schematic of the ωRNA. The crRNA-like region, tracrRNA-like region, and natural tetraloop are colored red, orange, and grey, respectively. The disordered regions are enclosed in dashed boxes.
