Extended Data Fig. 2: Single-particle cryo-electron microscopy analysis.
From: Cryo-EM structure of the transposon-associated TnpB enzyme
(a) Size-exclusion chromatography profile of the TnpB–ωRNA–target DNA complex. The peak fraction (indicated by a black bar) was analyzed by SDS-PAGE and urea-PAGE, and then used for cryo-EM analysis. (b) A representative cryo-EM image of the TnpB–ωRNA–target DNA complex, recorded on a 300 kV Titan Krios with a K3 camera. (c) Single-particle cryo-EM image processing workflow. (d) Fourier shell correlation (FSC) curve for the 3D reconstruction. The gold-standard cutoff (FSC = 0.143) is marked with the black dotted line. (e) Direct distribution plot (Viewing distribution plot). (f) Direction 3DFSC plots calculated by 3DFSC processing Server (https://3dfsc.salk.edu/upload/info/). (g) Local-resolution cryo-EM density map.
