Extended Data Fig. 8: CIP2A-TOPBP1 does not associate with acentric extrachromosomal DNA (ecDNA) elements.
From: Mitotic clustering of pulverized chromosomes from micronuclei
a) DAPI-stained metaphase spreads showing abundant ecDNAs in PC3 cells but not control HeLa S3 cells. Scale bar, 5 μm. b) CIP2A and TOPBP1 are not recruited to mitotic chromosomes in PC3 cells with ecDNAs in the absence of DNA damage. Examples of untreated and irradiated PC3 and HeLa cells arrested in mitosis and immunostained for CIP2A or TOPBP1. Scale bar, 5 μm. c) Quantification of CIP2A and TOPBP1 foci in (b). Data represent the mean ± SEM; P = 0.8768 (ns) for CIP2A; from left to right, n = 24, 23, 15, and 14 mitotic cells; P = 0.1437 (ns) for TOPBP1; from left to right, n = 18, 21, 20, and 12 mitotic cells; P-values calculated by two-tailed t-test comparing non-irradiated PC3 and HeLa S3 cells.
