Extended Data Fig. 5: Simultaneous mutations in ZnF-B and Rht-B1b in Fielder mimic the effect of r-e-z deletion. | Nature

Extended Data Fig. 5: Simultaneous mutations in ZnF-B and Rht-B1b in Fielder mimic the effect of r-e-z deletion.

From: Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat

Extended Data Fig. 5

a, Comparison of the agronomic traits including spikelet number per spike, fertile spikelet number, grain number per spike, grain length and grain width between Fielder (harboring Rht-B1b) and rht1-bb single mutant. b, Comparison of cell lengths of longitudinal section of the fully elongated uppermost internodes collected at anthesis stage between Fielder and the rht1-bb mutant (n = numbers of parenchymatic cells). Scale bar, 20 μm. c, Comparison of cell lengths of the cross sections of the developing grains collected at 10 days after pollination between Fielder and rht1-bb mutant (n = numbers of pericarp cells). Scale bars are 500 μm for the upper panels, and 100 μm for the lower panels. d, Comparison of the developmental and yielding traits between Fielder and znf-bb single mutant. e,f, Comparison of plant height, spike morphology, and grain traits between Fielder and two Eama mutants: eama-bb (e) and eama-aabbdd (f). In a,d,e,f, n = numbers of biologically independent samples. In a–f, data are means ± s.d.; P values were calculated by a two-tailed Student’s t-test (** P < 0.01; * P < 0.05; ns, no significant difference); Scale bars are 10 cm for whole plant, 5 cm for spike, and 1 cm for grain.

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