Extended Data Fig. 6: ZnF positively regulates brassinosteroid signalling.
From: Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat
a, Comparison of coleoptile lengths between the znf-bb mutant and Fielder (n = 28 biologically independent samples). b, Comparison of relative coleoptile lengths in response to various concentrations of epi-brassinolide (eBL) treatments between Fielder and znf-bb mutant (From 0 to 10 μM, n = 45, 45, 44, 45, and 45 plants for Fielder; n = 48, 48, 46, 48 and 45 plants for znf-bb mutant). Scale bars, 5 cm. c,d, Sensitivity to brassinazole (BRZ, BR synthesis inhibitor) was reduced in the znf-aabbdd triple mutant compared to Fielder. The relative shoot (c) and root (d) lengths of znf-aabbdd mutants compared to those in Fielder treated with different concentrations of BRZ. In c, from 0 to 50 μM, n = 32, 37 and 48 plants for Fielder; n = 36, 35 and 55 plants for znf-aabbdd mutant. In d, from 0 to 50 μM, n = 28, 34 and 52 plants for Fielder; n = 35, 32 and 48 plants for znf-aabbdd mutant. Data in a–d are mean ± s.e.m. e, Heatmap shows the differentially expressed genes (DEGs) in a znf-aabbdd mutant (line 2) relative to those in Fielder. f, Gene Ontology (GO) analysis of the DEGs in e. Up stands for upregulated DEGs in the znf-aabbdd mutant; down stands for downregulated DEGs in znf-aabbdd. g, Heatmap shows the DEGs involving in gibberellic acid (GA) metabolism and signaling. h, Heatmap shows the DEGs related to BR signaling. DEGs in e,g,h are identified using P value < 0.05 and absolute Log2(Fold change, FC) > 1 as criteria; Colors represent log2-fold change comparing relative expression. i, Schematic representation of the GA metabolic pathway in higher plants. Bioactive GAs (GA1, GA3, GA4) are marked with green oval background. j, Comparison of the levels of two GA isoforms between Fielder and znf-aabbdd (#2). k, Comparison of the levels of castasterone (CS) and typhasterol (TY) between Fielder and znf-aabbdd (#2). In j,k, Data are means ± s.d. (n = 3 biologically independent samples); * P < 0.05; ** P < 0.01; ns, no significant difference (two-tailed Student’s t-test).
