Extended Data Fig. 8: ZnF is an evolutionally conserved plasma membrane (PM)-localized protein across plant species.
From: Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat
a, Phylogenetic analysis of ZnF and its orthologs in different plant species. b, Schematic representation of the protein structure of ZnF. Blue boxes represent the putative transmembrane (TM) domains; green boxes are coiled coil (CC) domains; yellow triangles are the RING domains in ZnF. c, Protein sequence alignment of the three homoeologs of ZnF proteins deduced from AA, BB, and DD subgenomes of wheat. d, Transient expression of ZnF-GFP fusion proteins in N. benthamiana leaf epidermal cells for subcellular localization of ZnF. The red fluorescent protein (RFP)-tagged PIP2 was used as the plasma membrane marker. Scale bars, 20 μm. e, Transient expression of ZnF-GFP fusion proteins in wheat protoplasts. Scale bars, 10 μm. In d,e, all experiment was repeated independently three times with similar results.
