Extended Data Fig. 12: Anti-proliferative effects of TSP-1 inhibition in glioblastoma is limited to activity-dependent mechanisms.
From: Glioblastoma remodelling of human neural circuits decreases survival
Representative confocal images from HFC glioma monoculture showing no significant change in proliferation (as measured by the number of human nuclear antigen (HNA)-positive cells co-labelled with Ki67 divided by the total number of HNA-positive tumour cells counted across all areas quantified) upon pharmacological TSP-1 inhibition using (32 µM) gabapentin (HFC: n = 7; LFC: n = 9 regions, 2 per group; P = 0.50). Red, HNA (human nuclei); white, Ki67. Scale bar, 30 µm. Data presented as mean ± s.e.m. P values determined by two-tailed Student’s t-test. NS, not significant.
