Extended Data Fig. 12: Related to Fig. 7. STRIPAK and JNK signaling mediate the hyperproliferation phenotype caused by PXo knockdown in ECs and in progenitors.
From: A phosphate-sensing organelle regulates phosphate and tissue homeostasis
a,b, Puc-lacZ staining of midguts expressing Luc RNAi or PXo RNAi in progenitors for 7d. c,d,e, Midguts expressing Luc RNAi or PXo RNAi in ECs for 7d were stained for phosphorylated p38 (p-p38) and the cell border marker Dlg. Midguts fed with Bleo food for 2d were included as a positive control. f, Mitosis of midguts expressing PXo RNAi alone (n = 14) or PXo RNAi together with Cka RNAi (VDRC line, n = 15), Mob4 RNAi (VDRC, n = 14), or Strip RNAi (VDRC, n = 14) in ECs for 8d. P values from bottom to top: 1.50E-6, 3.60E-6, 8.08E-6. g, Mitosis of midguts expressing PXo RNAi together with Luc RNAi (n = 14), Strip RNAi (BDSC line, n = 14), mts RNAi (BDSC, n = 12), or Pp2A-29B RNAi (BDSC, n = 14) in ECs for 8d. P values from bottom to top: 1.86E-9, 5.40E-10, 3.69E-11. h, Mitosis of midguts expressing PXo RNAi together with Luc RNAi, Cka RNAi (BDSC), Mob4 RNAi (2 different BDSC lines), or bsk RNAi in progenitors for 9d. From left to right: n = 9, 10, 9, 11, 10; P = 1.07E-12, 3,33E-10, 4.93E-13, 2,72E-12. i, Mitosis of midguts expressing PXo RNAi alone (n = 12), or PXo RNAi together with Cka RNAi (VDRC line, n = 12), Mob4 RNAi (VDRC, n = 10), or Strip RNAi (VDRC, n = 12) in progenitors for 9d. P values from bottom to top: 4.57E-9, 3.72E-6, 4.57E-9. j, Mitosis of midguts expressing PXo RNAi together with Luc RNAi (n = 15), Strip RNAi (BDSC, n = 15), mts RNAi (BDSC, n = 14), or Pp2A-29B RNAi (BDSC, n = 12) in progenitors for 9d. P values from bottom to top: 6.29E-6, 2.07E-8, 1.72E-7. Data are mean ± s.e.m. P values are from one-way ANOVA with Bonferroni’s multiple comparison test. Scale bars, 50 µm.
