Extended Data Fig. 6: Pex5-cargo partitions into Pex13-IDR condensates.
From: Peroxisome biogenesis initiated by protein phase separation
Titration of a, Pex5 and b, mCherry-SKL to determine the concentration at which they do not form condensates. 1 µM of both Pex5 and mCherry-SKL were used for in vitro reconstitution assays with Pex13-IDR. c, Reconstitution experiments to measure partitioning of Pex5 labelled with AZ 647 dye and mCherry-SKL into Pex13-IDR WT condensates immediately after mixing at room temperature. d, Cas9-AZdye 488 maleimide does not actively partition into Pex13 droplets (top) while mCherry-SKL partitions with Pex5 and Pex13 (bottom). e, Pex5 and mCherry-SKL partition into Pex13-IDR WT (10 µM) and Pex13-IDR S8 (30 µM) condensates. f, Pex13-IDR S15 does not form spherical condensates. mCherry-SKL remains diffuse in most images of Pex13-S15 aggregates (top). In some images, however, Pex5-mCherry-SKL appears to partition with Pex13-S15 (bottom). This is likely due to experimental variation, perhaps a somewhat lower concentration of Pex5-mCherry-SKL, co-aggregating with Pex13-IDR-S15. Scale bar 5 µm.
