Extended Data Fig. 1: Clones for detection of soL1Rs.
From: Widespread somatic L1 retrotransposition in normal colorectal epithelium
a, A scatter plot showing mean sequencing coverage of clones and peak VAF of somatic mutations. Most clones showed their peak VAFs around 0.5, indicating that they were established from a single founder cell. b, Chromosome level copy number changes of the 887 normal clones. No significant genome-wide aneuploidy was detected, supporting genomic stability during clonal expansion of normal single cells. c, A Venn diagram showing the number of soL1Rs detected by each bioinformatics tool. d, A schematic plot describing two genomic footprints of retrotransposition, the poly-A tail and target site duplication. RT body, retrotransposed body; TSD, target site duplication. e, The distribution of target site lengths at insertion sites. Positive and negative target site lengths indicate target site duplication and deletion, respectively. soL1R, somatic L1 retrotransposition. f, Number of structural variations in 406 clones from normal colon epithelial cells. T-T inversion, tail-to-tail inversion; H-H inversion, head-to-head inversion.
