Extended Data Fig. 3: The dependence of key crotonyl-CoA producing enzymes in GSCs.
From: Lysine catabolism reprograms tumour immunity through histone crotonylation
a, RT-qPCR analysis of key crotonyl-CoA-producing enzymes in paired GSCs and DGCs. b,c, Expression levels of indicated genes from public datasets. n = 8 biologically independent cells in NSCs and GSCs from GSE119834. Three GSCs (MGG4, MGG6 and MGG8, each cell contains 3 replicates) and matched DGCs were queried in GSE54791. d–g, IB analysis (d,f) and cell proliferation (e,g) in DGCs (d,e) or NSCs (f,g) with or without GCDH KD. h,i, RT-qPCR analysis (h) and intracellular acyl-CoAs (i, n = 4 biologically independent samples) in GSC23 with or without indicated gene depletion. j, Relative intracellular SCFAs (n = 4 biologically independent samples) in GSC23 with or without GCDH KD cultured in media with indicated concentrations of l-lysine. Data are presented from three independent experiments in a,e,g and h. Representative of two independent experiments in d and f. Data are presented as mean ± SEM in a,e and g–j. Boxes represent data within the 25-to-75 percentiles in b and c. Whiskers depict the range of all data points. Horizontal lines within boxes represent mean values. Two-tailed unpaired t test for a–c and h, two-way ANOVA followed by multiple comparisons with adjusted p values for e and g, one-way ANOVA followed by multiple comparisons with adjusted p values for i and j. ns, not significant.
