Fig. 3: Pre-miR-155-RIBOTAC selectively degrades pre-miR-155 and reduces lung colonization in vivo.
From: Programming inactive RNA-binding small molecules into bioactive degraders
a, Left, proteome-wide changes in MDA-MB-231 cells treated with pre-miR-155-RIBOTAC (100 nM) versus vehicle (n = 3 biological replicates). Right, pre-miR-155-RIBOTAC significantly upregulated miR-155 related proteins (n = 98 proteins), as indicated by a Kolmogorov–Smirnov analysis (right) of their levels versus all proteins (n = 3 biological replicates). b, The effect of pre-miR-155-RIBOTAC on MDA-MB-231 cell migration (n = 3 biological replicates); 2 fields of view were quantified per replicate. Scale bars, 0.5 mm. c, pre-miR-155-RIBOTAC suppresses lung colonization in vivo, as determined by counting lung nodules (n = 5 mice) and by haematoxylin and eosin (H&E) staining (n = 5 mice; 2 fields of view were quantified per replicate). Scale bars, 1 mm (left) and 0.2 mm (right). d, The effect of pre-miR-155-amide-binder and pre-miR-155-RIBOTAC on pre-miR-155 levels in vivo, as determined by RT–qPCR using primers selective for human pre-miR-155 (n = 3 mice). Data are mean ± s.d. (b and d). Statistical significance was determined using a Wald’s test (a) or two-tailed Student’s t-tests (b–d).
