Extended Data Fig. 1: Cryo-EM data analysis of the NPC-trapped pre-60S ribosome.
From: Nuclear export of pre-60S particles through the nuclear pore complex
a, A representative raw micrograph of the pre-60S containing NPC particles from similar 187,076 micrographs and representative 2D class averages of pre-60S are shown on the right from selected 279,900 particles. b, Local resolution map for a central slice (left) and the overall 3D reconstruction (right). c, Angular distribution of particles used for the 3D reconstruction. d, FSC curves for the cryo-EM map of NPC-trapped pre-60S particle. The threshold of 0.143 was used to determine the overall resolution of the map. e, Flowcharts for cryo-EM data processing. See “Methods” for details.
