Extended Data Fig. 7: LCN2 is a mammokine involved in blocking cold-induced adipocyte UCP1 expression.
From: Mammary duct luminal epithelium controls adipocyte thermogenic programme
a) Real-time qPCR of indicated genes from mgWAT of AAV-GFP or AAV-LCN2 treated Lcn2 KO mice. N = 4,4. b) Gross appearance and H&E section of mgWAT from RT and cold-exposed LCN2KO mice (Top) and WT or LCN2KO mice exposed to 24 h cold (Bottom). Scale bar at 100 μm. c) Real-time qPCR of indicated genes from 24 h cold exposed females (Top) and males (bottom) WT and LCN2KO scWATs. N = 6,6. d) Intraductal injection of Ad-GFP or Ad-LCN2 contralaterally in the 4th and 5th nipple and immunofluorescence microscopy of GFP or LCN2 from Ad-GFP or Ad-LCN2 injected mice with indicated viral concentrations and barplot showing intensity of GFP or LCN2 from microscopy. Scale bar at 100 μm. Yellow asterisks indicate images also used in Main Fig. 4g. Representative image of two independent injections. For bar plots, quantification of intensity was calculated as a mean from multiple ducts from 1 image except for 2X1010 PFU for Ad-GFP (N = 3) and 2X109 for Ad-LCN2. (N = 2). Unpaired two-tailed Student’s t test *, p < 0.05; **, p < 0.01; ***, p < 0.001. Data are represented as mean ± S.E.M. Cartoon model in D created using BioRender.
