Extended Data Fig. 2: SNS fibers directly innervate ductal epithelial cells.
From: Mammary duct luminal epithelium controls adipocyte thermogenic programme
a) Light sheet microscopy fluorescence (LSFM) images of mgWAT isolated from female mice exposed to RT or COLD for 24 h and stained with TH antibody (SNS fibers) and EPCAM antibody (ductal cells). N = 6,6. Scale bar at 200 μm. b) Confocal images of mgWAT isolated from female mice exposed to RT or COLD for 24 h and stained for EPCAM and TH antibodies. Merged staining of EPCAM and TH represent neuroductal points. Representative images of 6 mice per condition. Scale bar at 100 μm. c-j) Quantification of indicated parameters of images from (b). N = 6,5. Unpaired Student’s t Test (j). Data are represented as mean ± S.E.M.
