Extended Data Fig. 8: Assessing the impact of CHD1L knock-out in primary monocyte-derived macrophages on HIV infection.
From: Africa-specific human genetic variation near CHD1L associates with HIV-1 load
a, CHD1L was efficiently knocked out in primary MDMs by 3 of 5 crRNP constructs and a combined, multiplexed pool. b, Percent infected cells 4 days post-challenge as measured by flow cytometry showed an increase in three of the four CHD1L knockout pools compared to the non-targeting control, but these differences were not statistically significant. c,d, p24 levels in the culture supernatants as measured by ELISA were lower in CHD1L knockout cell pools 2 days post-infection (c), but recovered to the level of the non-targeting control by 4 days post-infection (d).
