Extended Data Fig. 2: Single-particle reconstruction of the NTSR1–GRK2–Gαq complex.

a, Flowchart of cryo-EM data analysis of the NTSR1–GRK2–Gαq complex. b, Micrograph of the reference-free 2D class averages. c-d, Global Cryo-EM maps of the NTSR1–GRK2–Gαq complexes were generated and colored by local resolutions from 2.4 Å (blue) to 4.5 Å (red). e-g, Local Cryo-EM maps focusing on NTSR1, GRK2 bound with Gαq and only Gαq protein were generated and colored by local resolutions from 2.4 Å (blue) to 4.5 Å (red) respectively. h-l, The “Gold-standard” Fourier shell correlation (FSC) curve indicates that the resolution of the global electron density map of the NTSR1–GRK2–Gαq complex 1 is 3.08 Å (h), the NTSR1–GRK2–Gαq complex 2 is 3.10 Å (i), and the resolution of the local electron density maps of NTSR1 (contains a partial of GRK2), GRK2 bound with Gαq and only Gαq protein from complex 1 are 2.91 Å (j), 2.81 Å (k) and 3.02 Å (l), respectively.