Extended Data Fig. 5: Postnatal transitional cells express early basal cell markers.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a, Bar plots demonstrating the composition of epithelial subclusters in each individual mouse (n = 8 mice). The transitional cell cluster was consistently expanded in all four NDUFS2 cKO mice compared with NDUFS2 control mice. M, male; F, female. b, Heatmap showing expression of hallmark identifier genes for each epithelial cell type. Early basal cell marker genes (Krt8, Krt18, Krt7, Krt19) are highly expressed in transitional cells and some of the AT1 cluster. c, Cell-cycle score analysis of epithelial cells was performed and plotted on a UMAP embedding. Cells predicted to be in G0/G1, G2/M, and S phases are shown in separate UMAPs, respectively. No subcluster of epithelial cells was predicted to be in a specific cell-cycle phase. d, Volcano plots visualizing the differential gene expression results by mouse genotype in the AT1 cluster from single-cell RNA sequencing analysis. x axis shows average log2 fold change, and y axis shows −log10 false discovery rate (FDR) q value. e, UMAP embedding of AT1 cells (n = 723 cells) colored by subcluster. f, UMAP plot depicting AT1 cell origins with respect to the mouse genotype. g, Bar plots demonstrating the composition of AT1 subclusters in NDUFS2 control and NDUFS2 cKO mice. h, Heatmap showing expression of epithelial marker genes in AT1 subclusters. Cells in the AT1_1 cluster express higher level of transitional cell marker genes compared to those in other AT1 subclusters.
