Extended Data Fig. 7: Postnatal transitional cells are characterized by increased ISR.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a, Gene set enrichment analysis of top gene signatures that are up-regulated (blue) or down-regulated (red) in lung epithelial cells from NDUFS2 cKO mice (n = 7) compared to NDUFS2 control mice (n = 8). FDR ≤ 0.05. b, Enrichment plot of the ISR signature genes in lung epithelial cells from NDUFS2 cKO mice (n = 7) compared to NDUFS2 control mice (n = 8) (normalized enrichment score; 2.80, false discovery rate q value <0.0001). c, Expression levels of Atf genes in epithelial subclusters are plotted in violin plots. d, Violin plots of ISR enrichment scores across all the cell clusters. p < 2.2 × 10−16 by Kruskal-Wallis test. Transitional cells have more enriched ISR gene signature than any other cell types (*** adjusted p < 2.0 x 10−16 by post-hoc pairwise Mann-Whitney test with Holm method, p values are in the Source Data). e, Heatmap of ISR signature genes by epithelial subclusters. Results are from RNA-sequencing analysis of lung epithelial cells isolated from fifteen 35-day-old mice (a–b, related to Fig. 4a-b), and single-cell RNA sequencing analysis from eight 21-day-old mice (c–e).
