Extended Data Fig. 13: Loss of mitochondrial complex II in lung epithelial cells induces mild ISR, which is lower than the ISR induction due to loss of mitochondrial complex I.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a, The frequency distribution of alveolar thickness measured in hematoxylin-eosin stained lung histology of 47–49 day-old mice (n = 4 mice, two males and two females per genotype). 4–6 randomly selected fields of view from each mouse were evaluated. The x axis shows alveolar thickness bins, and the y axis shows the number of alveolar pixels that belong to the respective alveolar thickness bin normalized to the total alveolar pixel count in the image. Each animal is represented by its own color. Statistical significance for genotype was calculated based on F-test for a linear model (p = 4.65 x 10−3). b, RNA-seq analysis of lung epithelial cells from 35-day old mice (SDHD control n = 6; SDHD cKO n = 7; NDUFS2 control n = 8; NDUFS2 cKO n = 7 mice). Heatmap of ATF transcripts in lung epithelial cells. c, Relative abundance of succinate in lung epithelial cells isolated from 35-day old mice (NDUFS2 control n = 8; NDUFS2 control/NDI1 n = 7; NDUFS2 cKO n = 7; NDUFS2 cKO/NDI1 n = 8 mice). d, Relative abundance of succinate in lung epithelial cells isolated from 35-day old mice (NDUFS2 control n = 13; NDUFS2 control + ISRIB n = 8; NDUFS2 control + NMN n = 9; NDUFS2 cKO n = 7; NDUFS2 cKO + ISRIB n = 9; NDUFS2 cKO + NMN n = 9; WT n = 6 mice). Lines represent median. p = 0.2333 by Kruskal-Wallis test.
