Fig. 2: Expression of the yeast NDI1, an alternative NADH dehydrogenase, in lung epithelial cells reverses abnormal postnatal alveolar development in NDUFS2 cKO mice.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a, Schematic of the mitochondrial ETC with ectopic NDI1. b,c, Metabolomics analysis of lung epithelial cells isolated from 35-day-old mice (NDUFS2 control n = 8; NDUFS2 control/NDI1 n = 7; NDUFS2 cKO n = 7; NDUFS2 cKO/NDI1 n = 8 mice). b, Relative abundance of lactic acid. Lines represent median. P = 0.0013 by Kruskal–Wallis test b. c, The heat map displays the relative abundance of significantly changed metabolites c. α-KG, α-ketoglutarate; AICA, 5-aminoimidazole-4-carboxamide; DHAP, dihydroxyacetone phosphate; DOPAL, 3,4-dihydroxyphenylacetaldehyde; F6P, fructose-6 phosphate; G1P, glucose-1 phosphate; G6P, glucose-6 phosphate; GAP, glyceraldehyde 3-phosphate; IMP, inosine monophosphate; l-NMMA, NG-monomethyl-l-arginine; XMP, xanthosine monophosphate. d, Survival of NDUFS2 cKO (n = 12) and NDUFS2 cKO/NDI1 (n = 22) mice (P < 0.0001 by log-rank test). e, Representative images of littermates’ lung histology (haematoxylin and eosin stain) in 46-day-old mice. Scale bar, 50 μm. f, Box plots of lung compliance of 46- to 50-day-old mice (NDUFS2 control n = 21; NDUFS2 cKO n = 11; NDUFS2 cKO/NDI1 n = 12 mice with technical replicates). P = 0.0016 by one-way analysis of variance. Adjusted P values by Šídák’s multiple comparisons test in the graph. a.u., arbitrary units.
