Fig. 3: A distinct epithelial population of transitional cells emerges during postnatal lung development in NDUFS2 cKO mice.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a, Uniform manifold approximation and projection (UMAP) plot showing single-cell RNA-seq analysis of 57,886 cells isolated from 21-day-old mouse lungs (n = 4 mice (two males and two females) in each genotype). aCAP, alveolar capillary endothelial cells; Art, arterial endothelial cells; B, B cells; CM, classical monocytes; DC.1, dendritic cells 1; DC.2, dendritic cells 2; FIB.1, fibroblasts 1; FIB.2, fibroblasts 2; gCAP, general capillary endothelial cells; ILC, innate lymphoid cells; IM, interstitial macrophages; lymph EC, lymphatic endothelial cells; MP, macrophages; NCM, non-classsical monocytes; NK, natural killer cells; Pl.DC, proliferating dendritic cells; Pl.MP, proliferating macrophages; SM, smooth muscle cells; T, T cells; Treg, regulatory T cells (Extended Data Table 1). b, UMAP plot depicting cell origins with respect to the mouse genotype. c, UMAP plot showing the expression of a Sftpc lineage tracing fluorescent marker, tdTomato. Darker colour represents higher expression. d, UMAP embedding of lung epithelial cells (n = 9,322 cells) coloured by cell type. CiliaSecretory, club cells and ciliated cells. e, UMAP plot depicting epithelial cell origins with respect to the mouse genotype. f, Bar plots demonstrating the composition of epithelial subclusters in cells from NDUFS2 control and NDUFS2 cKO mice. g, Marker gene expression by epithelial cell type in each mouse genotype is displayed in a dot plot, where the size of the dot indicates the proportion of cells within the cell type expressing that gene and higher expression is represented as a darker colour. In this dot plot, AT2 and AT2-Lyz1+ clusters and Transitional and Transitional-Lyz1+ clusters were merged into ‘AT2’ and ‘Transitional’, respectively.
