Extended Data Fig. 2: Global analysis of translational dynamics and dual-luciferase reporter study of uAUG-containing transcripts.
From: Pervasive downstream RNA hairpins dynamically dictate start-codon selection
a, Flow chart of RNA-seq and Ribo-seq data analysis. b, Strategy for the identification of translating mAUGs and uAUGs (see Methods for details). c, Dual-luciferase reporter study (top) of translational responses of the 5′ leader sequences of 20 TE-up transcripts to elf18 induction (bottom). FLUC reporter without the inserted test sequence was used as a negative control (Neg Ctl). P values were calculated by two-tailed Student’s t-test. Values are mean ± s.e.m. (n = 5 biological replicates). d,e, GO enrichment analysis on the 1,157 TE-up transcripts (d) and 1,150 TE-down transcripts (e). The size of the dot represents the number of genes that fall into each group. The colour of the dot represents adjusted P value.
