Extended Data Fig. 8: Functional annotation of NMPFs with remote structural homologues.

Five example NMPFs (a-e) are shown. Annotation is performed using using structural information (left), gene co-occurrence analysis (middle), and ecosystem distribution (right). Each of the NMPFs has a high-quality 3D model with at least one remote structural homologue to SCOPe. The NMPFs’ 3D models, produced with AlphaFold, and the structures of the SCOPe domains are rendered in the same orientation and coloured based on their per-residue structure confidence (pLDDT for AlphaFold models and inverse B-factor for experimental structures). The gene neighbourhood of each NMPF is presented in the form of an association network; with nodes representing gene products (the NMPFs and their adjacent genes that encode Pfam domains) and edges representing co-occurrence in the same sequencing scaffold. Pfam domains are further grouped using their associated COG functional categories as annotation. Finally, the NMPFs’ associated ecosystems are presented in pie charts. Ecosystems with a <1% presence in the NMPFs are summed into the category “Other ecosystems”.