Extended Data Fig. 4: Caspase activation is not involved in SASP regulation in senescent cells.
From: Apoptotic stress causes mtDNA release during senescence and drives the SASP
(a) Scheme showing the mechanism of miMOMP-induced caspase activation. (b) Western blot showing successful CRISPR/Cas9 deletion of APAF1 in proliferating and senescent (IR) MRC5 human fibroblasts. Representative of n = 2 experiments. (c) Graph showing population doublings of EV and APAF1-deficient cells. Data are representative of n = 2 independent experiments. Quantification of mRNA levels of (d) p16INK4A, (e) p15, (f) p21 (n = 6 independent experiments). (g) Quantification of the percentage of EV and APAF1 deficient cells positive for Ki67. Each dot represents the average per image (n = 3 technical replicates; 6 images taken per replicate). Quantification of mRNA levels of (h) IL-6, (i) IL-1β, (j) IL-8, (k) MCP1 in proliferating and senescent (IR) EV and APAF1-deficient cells (n = 6 independent experiments). Data are mean ± S.E.M. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test (d-k). For gel source data (b), see Supplementary Fig. 1.
