Extended Data Fig. 6: Expression of a tamoxifen-inducible viral DNase (HSV-1 UL12.5) that specifically targets mitochondria, results in depletion of mtDNA and suppresses the SASP.
From: Apoptotic stress causes mtDNA release during senescence and drives the SASP
a) Relative mtDNA copy number of MRC5-UL12.5 fibroblasts with and without tamoxifen treatment (100 nM, 48 h). (b-e) mRNA expression levels of SASP genes, IL6, IL8, Ccl2 and IL-1α in proliferating and senescent (IR) MRC5-UL12.5 fibroblasts with and without tamoxifen treatment (48 h). Data are mean ± S.E.M. of n = 3 independent experiments. Statistical significance was assessed using two-sided Student’s unpaired t-test (a), one-way ANOVA followed by Tukey’s multiple comparison test (b-e).
