Extended Data Fig. 3: Testing the toxicity of putative TumA antitoxins.
From: Uncovering new families and folds in the natural protein universe
Antitoxin expression plasmids were cotransformed with empty toxin expression vectors (pBAD33) into E. coli BW25113 cells. The bacterial cultures were started from a single colony and grown for five hours in liquid LB media supplemented with appropriate antibiotics. The cultures were normalised to OD600 = 1.0, serially diluted and spotted on LB agar plates containing appropriate antibiotics and 500 µM IPTG for antitoxin induction and 0.2% arabinose to mimic the conditions in toxin neutralization assay. The experiment was made in n = 3 biologically independent replicates.
