Extended Data Fig. 4: Superimposition of Crt-SPARTA with TtAgo and human Ago2.
From: Nucleic-acid-triggered NADase activation of a short prokaryotic Argonaute
a, The structure of the Crt-SPARTA heterodimer is superimposed with the structure of TtAgo complexed with gDNA and tDNA (PDB: 4N47) b, The structure of the Crt-SPARTA heterodimer is superimposed with the structure of human Argonaute 2 bound to a gRNA and tRNA (human Ago2, PDB: 4W5O). Crt-SPARTA is coloured by chain. Crt-TIR-pPAZ is coloured light blue, Crt-Ago is coloured magenta; individual domains of Crt-SPARTA are labelled. TtAgo and human Ago2 are coloured grey and the guide DNA or RNA and target DNA or RNA are coloured orange and red, respectively. Crt-pPAZ lacks the PAZ ___domain but retains an N-like and a linker ___domain, topologically equivalent to the N and L1–L2 domains of TtAgo and human Ago2. Crt-pPAZ contains a unique C-terminal trigger ___domain unseen in previous Ago structures. The trigger ___domain seals the exit of the target DNA–RNA-binding tunnel.
