Extended Data Fig. 5: Postsynaptic neurons of R8s.
From: A single photoreceptor splits perception and entrainment by cotransmission
a, PA-GFP labeling of AMA neurons postsynaptic to R8s. Left, tdTomato is used to identify the dorsal commissure (yellow dashed box) in VT037867-Gal4,UAS-tdTomato,UAS-PA-GFP flies for photoactivation (top), PA-GFP-labeled cell bodies and commissure of AMA neurons (middle), merged image (bottom); right, negative control of PA-GFP labeling: tdTomato-labeled dorsal commissure track in VT037867-Gal4,UAS-tdTomato,UAS-PA-GFP flies (top), no PA-GFP-labeling without photoactivation (middle), merged image (bottom). b, pooled total number of PA-GFP-labeled AMA neurons. Pooled data: mean ± s.e.m. c, aMe 12 belong to AMA neurons. Left, Co-labeling of aMe 12 (SS01050 > RFP) and AMA neurons (VT037867-LexA > GFP); right, an expanded view of the dashed boxes. d, Ort-expressing neurons postsynaptic to R8s. Overlap of anterograde tdTomato labeling with trans-Tango and ort-LexA > GFP revealed ort-expressing postsynaptic neurons downstream of pR8s (top) and yR8s (bottom). e, Histamine-responsive neurons postsynaptic to R8s. GFP expression is intersected by ort-LexA > FLP and L1, Tm5, Tm9, or Tm20-spGal4. f, GRASP between R8s and L1, Tm5, Tm9, and Tm20.
