Extended Data Fig. 7: Clock neurons are postsynaptic to AMA neurons.
From: A single photoreceptor splits perception and entrainment by cotransmission
a, Clock neurons overlap with postsynaptic neurons of AMA neurons. Double-labeling clock neurons (with anti-TIM and anti-PDF) shows overlap with tdTomato-labeled postsynaptic neurons of AMA neurons (VT037867-Gal4>trans-Tango). b, An expanded view of the white rectangles (top) and the dashed boxes (bottom) in a. Arrow: LNds; asterisk: 5th s-LNv; arrowhead: l-LNv; unfilled arrowhead: s-LNv. c, Evening clock neurons are postsynaptic to AMA neurons. Top, Co-labeling ITP-LNd and 5th s-LNv (R54D11-LexA > GCaMP7s) and postsynaptic neurons of AMA neurons (VT037867-Gal4>trans-Tango); bottom, an expanded view of the white boxes in top panels. Arrow: LNds; asterisk: 5th s-LNv. d, Varied synaptic strengths between AMA neurons and clock neurons. Left, schematic of dual-patch recordings; middle, representative responses of a pair of ITP-LNd and s-LNv to optogenetic activation of AMA neurons; right, pooled peak responses (lines represent pairs of neurons simultaneously recorded with dual patch-clamp recordings). Light: 617 nm, 2 ms, 2.22 × 109 photons/μm2/s. e, R8s differentially excite different clock neurons. Left, representative R8-driven light responses of different clock neurons; right, pooled peak response amplitudes (mean ± s.e.m.). Light: 470 nm, 2 ms, 5.65 × 107 photons/μm2/s. Pooled data are mean ± s.e.m. *P < 0.05; **P < 0.01. Statistical analysis is summarized in Supplementary Table 2.
