Extended Data Fig. 1: R8-mediated irradiance signals.
From: A single photoreceptor splits perception and entrainment by cotransmission
a, PLC-dependent, but histamine- and cryptochrome-independent light responses. Top, Representative light-triggered currents of ITP-LNd in norpAP41 (left) and CHO (right) flies; middle, representative light-triggered depolarization; bottom, pooled data. Light: 470 nm, 5.65 × 107 photons/μm2/s, 2 ms (top) and 2 s (middle). b, H-B eyelet ablation in Rh6-hid,rpr flies on day 3 after eclosion. c, Time-dependent H-B eyelet ablation by Rh6-hid,rpr. Top, H-B eyelet axons and yR8s on different days after eclosion; middle, top views of yR8 loss; bottom, probability of H-B eyelet existence (left), and pooled data of yR8 counts (right). Arrow: H-B eyelet axons. d, Histamine-independent light responses remained in the absence of H-B eyelets. Top, schematic of laser cutting H-B axons, middle, representative ITP-LNd responses; bottom, pooled data. Arrow: intact GFP-labeled H-B eyelet axons; arrowhead: a cavitation bubble associated with laser-ablation of H-B eyelet axons. Light stimulation: 470 nm, 2 ms, 5.65 × 107 photons/μm2/s. e-g, Specificity and completeness of laser cutting H-B eyelet axons. e, Top, representative H-B eyelets and yR8s (left), and their calcium response to light stimulation (right); bottom, laser cutting of H-B eyelet axons (left), and representative light-triggered calcium responses. Light stimulation: 460 nm, 2 s, 1.18 × 106 photons/μm2/s. White rectangle: axon bundle of H-B eyelets; arrow: a cavitation bubble associated with laser ablation. f, Representative calcium responses of yR8s (top) and H-B eyelets (bottom). Light stimulation: 460 nm, 2 s, intensities of 0.03, 0.30, 0.97, 1.18, and 2.36 × 106 photons/μm2/s. Blue bars: timing of light stimulation. g, Pooled calcium responses of yR8s (n = 5 flies) and H-B eyelets (n = 5 flies). Light stimulation: 460 nm, 2 s, 1.18 × 106 photons/μm2/s. h, Histamine-independent responses after photoreceptor silenced by TNT. Pooled data: mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant. Statistical analysis is summarized in Supplementary Table 2.
