Extended Data Fig. 6: Differential cell type abundance across T-box groups in the mesoderm and spinal cord neurons.

a, A subset of trunk muscle and spinal cord neuron cell types for each of four perturbations relative to control embryos at matched timepoints: tbx16, tbx16; msgn1, tbx16; tbx16l, and tbx16^−/−. Black boxes indicate significance (q value < 0.01, beta-binomial regression with multiple testing correction; n.d. - no cells of this type detected at these stages). The mean percent of each cell type per whole embryo is represented by an additional color bar. b, A prediction of our ability to assign significance to cell type abundance changes across effect sizes and cell type proportions. The model is using the reference data as input and a Dirichlet multinomial distribution; p-values are assigned using our beta-binomial regression approach. Here, significant cell types are those with a p value < 0.05 (beta-binomial regression), points that fall below the threshold are black.