Extended Data Fig. 8: High glucose impairs lung DC immune functions.

a-d, WT (n = 9) and Akita mice (n = 6) infected with 50 pfu PR8 and intratracheally administered 100μg OVA-AF647. a, Representative flow cytometry, lung dLN OVA⁺DC. b, Percentage of lung dLN OVA⁺cDC2, two-sided unpaired t-test. c, Number of lung dLN OVA⁺DC, two-sided unpaired t-test. d, Frequency of lung OVA⁺DC, WT (n = 10) and Akita (n = 7) mice, cDC1 and CD64⁺ DC, two-sided unpaired t-test, cDC2, Mann Whitney U-test. e-g, Lung dLN cDC2 MFI, WT (n = 9) and Akita (n = 6) mice, two-sided unpaired t-test. e, CD40. f, CD80. g, CD86. h-j, Lung DC MFI, WT (n = 10) and Akita (n = 7) mice. h, CD40, two-sided Mann Whitney U-test. i, CD80, two-sided unpaired t-test. j, CD86, two-sided unpaired t-test. k, WT Lung DC incubated with high (50mM, n = 4) or normal (10mM, n = 4) glucose, cell viability, two-sided unpaired t-test. l-n, WT Lung cDC2 incubated with high (50mM, n = 4) or normal (10mM, n = 4) glucose, MFI, two-sided unpaired t-test. l, CD40. m, CD80. n, CD86. o-t, WT Lung DC incubated with 25 mM (n = 10) or 5 mM (n = 10) glucose, two-sided Mann Whitney U-test for p, two-sided unpaired t-test for all other panels. o, cDC1 CD40. p, cDC1 CD80. q, cDC1 CD86. r, cDC2 CD40. s, cDC2 CD80. t, cDC2 CD86. u-ab, WT and Akita mice intratracheally administered 100μg HDM + 100 μg OVA-AF647.u, Lung dLN OVA⁺DC, WT (n = 8) and Akita (n = 7) mice, two-sided unpaired t-test. v-x, Lung dLN DC MFI, WT (n = 10) and Akita (n = 5) mice, two-sided unpaired t-test. v, CD40. w, CD80. x, CD86. y, Lung OVA⁺DC frequency, WT (n = 10) and Akita (n = 7) mice, two-sided Mann Whitney U-test. z-ab, Lung DC MFI, WT (n = 9) and Akita (n = 5) mice. z, CD40, two-sided unpaired t-test. aa, CD80, two-sided Mann Whitney U-test. ab, CD86, two-sided Mann Whitney U-test. All data mean+s.e.m.