Extended Data Fig. 10: Lung DC from diabetic animals induce an attenuated T cell response.

a-h, WT and Akita mice intratracheally administered 100μg HDM, followed, 24h later, by DC sorting and transfer to WT recipients (receiving WT DC (n = 9), Akita DC (n = 10), or No DC (n = 7)). Recipient mice intratracheally challenged by 10μg HDM daily from day 7-11, analyzed at day 14. a, GATA3⁺CD4⁺ T cells, Kruskall Wallis test and Dunn’s correction. b, Ki-67⁺ T cells, Kruskall Wallis test and Dunn’s correction. c, Frequency of lung IL-10⁺CD4⁺ T cells, one-way ANOVA and Holm-Sidak correction, and IL-17A⁺CD4⁺ T cells, Kruskal Wallis test with Dunn’s correction. d, Frequency of lung RORγt⁺CD4⁺ T cells, T-bet⁺CD4⁺ T cells, Kruskal Wallis test with Dunn’s correction, and FoxP3⁺CD4⁺ T cells, one-way ANOVA and Holm-Sidak correction. e-g, WT and Akita mice intratracheally administered UV-inactivated PR8, followed 20 h later, by DC sorting and trasnfer to WT recipients (receiving WT DC (n = 6), WT+Akita DC (n = 6)). 10 days later, recipients infected with 500pfu PR8. e, Lung CD4⁺ T cells and CD8⁺ T cells, two-sided unpaired t-test. f, Frequency of T-bet⁺ cells, unpaired t-test. g, PR8 viral RNA, Mann Whitney test, WT+WT DC (n = 15), WT+Akita DC (n = 13), pooled from 2 experiments. h-o, WT lung DC incubated with high (50mM)/normal (10mM) glucose and 2-DG/vehicle for 20h, two-way ANOVA and Holm-Sidak correction. h, lung DC viability (n = 3/group). i-k, cDC1 (n = 4/group). i, CD40 MFI. j, CD80 MFI. k, CD86 MFI. l-n, cDC2 (n = 4/group). l, CD40 MFI. m, CD80 MFI. n, CD86 MFI. o, IL-12⁺ cells (n = 4/group). p-r, WT lung cDC1 incubated for 20 h with high (50mM, n = 12)/normal (10mM, n = 12) glucose, and high (50mM) glucose+2-DG (n = 3)/normal (10mM) glucose+2-DG (n = 3), then co-cultured with OT-I-CD8⁺ T cells for 4 days in normal (10mM) glucose (in the absence of 2-DG), two-way ANOVA and Holm-Sidak correction. p, CD8⁺ T cells. q, TNF⁺ cells. r, IFNγ⁺ cells. s-u, WT Lung cDC2 incubated for 20 h with high (50mM, n = 12)/ normal (10mM, n = 12) glucose, and high (50mM) glucose+2-DG (n = 3)/normal (10mM) glucose+2-DG (n = 3), then co-cultured with OT-I-CD8⁺ T cells for 4 days in normal (10mM) glucose (in the absence of 2-DG), two-way ANOVA and Holm-Sidak correction. s, CD8⁺ T cells. t, TNF⁺ cells. u, IFNγ⁺ cells. v, Mice intraperitoneally administered 250 mg/kg 2-DG (n = 5) or PBS (n = 5). Blood glucose measured at indicated time-points, two-sided unpaired t-test for each time-point. All data mean+s.e.m.