Extended Data Fig. 11: Diabetes does not impact lung DC lipid metabolism or glucose uptake.

a, Mice infected with 200pfu PR8 and intraperitoneally administered 250 mg/kg 2-DG (n = 10) or PBS (n = 10) daily. Survival, log-rank Mantel-Cox test. b-k, Mice infected with 50pfu PR8 and intraperitoneally administered 250 mg/kg 2-DG (n = 7) or PBS (n = 10) daily. b, Lung PR8 viral RNA, two-sided Mann-Whitney U-test. c, Lung virus-specific NP34 Tetramer⁺CD8⁺ T cells, two-sided unpaired t-test. d, Lung CD8⁺ T cells and CD4⁺ T cells, two-sided unpaired t-test. e, Frequency of IFNγ⁺CD8⁺ T cells, two-sided unpaired t-test. f, Lung Ki-67⁺CD8⁺ T cells, two-sided unpaired t-test. g, B cells,two-sided unpaired t-test. h, GC B cells, two-sided unpaired t-test. i, Lung cDC1, two-sided unpaired t-test. j, Lung cDC2, two-sided Mann-Whitney U-test and CD64⁺ DC, two-sided unpaired t-test. k, Lung Ki-67⁺cDC1, Ki-67⁺cDC2 and Ki-67⁺CD64⁺ DC, two-sided unpaired t-test. l-n, Mice intraperitoneally administered 250 mg/KG 2-DG (n = 10) or PBS (n = 10) daily for 10 days, two-sided unpaired t-test. l, Lung cDC1. m, Lung cDC2 and Lung CD64⁺ DC. n, Lung T cells. o-p, Seahorse ECAR analysis of lung DC, WT (n = 8) and Akita (n = 6) mice, two-sided unpaired t-test. q, Seahorse OCR of lung DC, WT (n = 12) and Akita (n = 8) mice, AUC, two-sided Mann-Whitney U-test. r-s, scRNA-seq of lung DC of genes involved in beta-oxidation. Heatmap shows z-scores of mean scaled and log normalized gene expression of cells in different DC. t, Lung DC incubated with 11 mM ¹³C-glucose. Intracellular pyruvate measured after 6 h (isotopologue fractional enrichment), 3 pooled experiments, two-sided paired t-test. All data mean+s.e.m. AUC, area under the curve; OCR, oxygen consumption rate.