Extended Data Fig. 5: Hyperglycaemia dysregulates the lung DC compartment in infection and homeostasis.

a-d, Mice infected with 50pfu PR8, treated with Ins/PBS: WT+PBS (n = 6), WT+Ins (n = 7), Akita+PBS (n = 6), Akita+Ins (n = 9). a-c, one-way ANOVA and Holm-Sidak correction, d, Kruskal Wallis test with Dunn’s correction. a, Lung cDC2. b, Lung CD64⁺ DC. c, Lung IL-12⁺cDC1. d, Lung Ki-67⁺cDC1. e-f, Naïve WT (n = 8) and Akita (n = 8) mice, two-sided unpaired t-test. e, Lung cDC1, cDC2 and CD64⁺ DC. f, Lung Ki-67⁺ DC. g-i, Naïve WT (n = 10) and Akita (n = 10) mice, two-sided unpaired t-test. g, BM DC precursors. h, BM Pre-DC subsets. i, Blood lymphocytes. j-l, WT (n = 15) and Db/Db (n = 10) mice infected with 50pfu PR8, pooled data from 3 experiments. j, Lung cDC1, two-sided Mann Whitney U-test. k, Lung cDC2 and CD64⁺ DC, two-sided unpaired t-test. l, Lung Ki-67⁺ DC, two-sided unpaired t-test for cDC1 and CD64⁺ DC, two-sided Mann Whitney U-test for cDC2. m-p, WT and Akita mice infected with 50pfu PVM (n = 7 per group in m-o, WT (n = 8) and Akita (n = 10) mice in p). m, Lung cDC2, two-sided unpaired t-test and CD64⁺ DC, Mann Whitney U-test. n, Lung pDC, two-sided Mann Whitney U-test. o, Lung Ki-67⁺ DC, two-sided unpaired t-test. p, Lung IL-12⁺cDC1, two-sided unpaired t-test. q-r, WT and Akita mice treated with insulin/PBS: WT+PBS (n = 16), WT+Ins (n = 18), Akita+PBS (n = 14) Akita+Ins (n = 14), pooled data from 2 experiments. q, Lung cDC1, Kruskal Wallis test with Dunn’s correction.r, Lung cDC2 and CD64⁺ DC, Kruskal Wallis test with Dunn’s correction. s-t, WT and Akita mice treated with insulin/PBS: WT+PBS (n = 6), WT+Ins (n = 9), Akita+PBS (n = 7) Akita+Ins (n = 7), one-way ANOVA and Holm-Sidak. s, Lung Ki-67⁺cDC1. t, Lung IL-12⁺cDC1. All data mean+s.e.m. CDP, Common dendritic cell progenitor; MDP, Macrophage dendritic cell progenitor.