Extended Data Fig. 5: Identification of novel cell types at spatial and single-cell level.
From: A human embryonic limb cell atlas resolved in space and time
a, The VisiumStitcher workflow for merging the 10x Visium spatial data of the human limb. b, Immunofuorescence staining of RUNX2, THBS2 and COL2A1 on the longitudinal section of the tibia from a PCW7 embryo. n = 3. Scale bar, 50 μm. c, Uniform manifold approximation and projection (UMAP) visualization of smooth muscle progenitor (SMProg), neural fibroblasts (NeuralFibro), pericyte and smooth muscle (SMC). d, Violin plot showing the expression level of FOXS1, PI16, FGF19, KCNJ8 and ACTA2 in SMProg, NeuralFibro, pericyte and SMC, using normalised and log-transformed values. e, Immunohistochemical staining of PI16 and FGF19 showing the NeuralFibro in the sciatic nerve at PCW9. The neurofilament was stained with NEFH antibody. A neighbouring section stained with H&E solution is also shown. n = 4. Scale bar, 50 μm. f, UMAP visualization of tendon progenitor (TenoProg), tenocytes (Teno) and perimysium cells. g, Violin plot showing the expression level of SCX, TNMD, GCG, BGN and KERA in TenoProg, Perimysium, and Teno. The expression level of genes is the normalised and log-transformed values. h, RNA-ISH (GCG) combined with immunohistochemistry (MYH3 and KERA) of tissue sections from human hind limb showing the spatial expression pattern of GCG, MYH3 and KERA. n = 2. Scale bar, 1 mm.
