Extended Data Fig. 7: The transcriptional regulation of LPM differentiation in the human limb.
From: A human embryonic limb cell atlas resolved in space and time
a, Heatmap illustrating the vertically normalised mean activity of selected transcription factors for each cell type from soft connective lineage of LPM. b, Force-directed graphs (top panel) and heatmaps across tissue section from the human hindlimb at PCW5.6 and PCW6.2 (bottom panel) showing the expression pattern of representative transcription factors in normalised and log-transformed values. c, Stacked bar chart showing the fraction of cell cluster per time point, coloured by cell type and grouped by tissue type. PCW, post conception week. SmM, smooth muscle group; other abbreviations as per Fig. 1. d, Dot plot showing the expression level of marker genes of osteochondral cell clusters. The colour bar indicates the mean normalised expression level. e, Force-directed graph of cells per time point, coloured by cell type in a. f, Uniform manifold approximation and projection (UMAP) visualization of the chondrocyte lineage with arrows representing inferred differentiation directions (See Methods). g, Stacked bar chart showing the fraction of phase of cell cycle per osteochondral cell cluster. h, Force directed graph showing the expression level of SCX and SOX9. The colour bar indicates the normalised and log-transformed expression values. i, Scatter plots showing the expression level of SCX and SOX9 expression in all LPM-derived cells in normalised and log-transformed values. The percentages of double positive cells are given. j, Heatmap across tissue section from the human hindlimb at PCW6.2 showing SCX and SOX9 expression in normalised and log-transformed values. The voxels marked with yellow asterisks express both SCX and SOX9. k, RNA-ISH of tissue sections from the human hindlimb showing the expression of SCX and SOX9 in situ.
