Fig. 4: Cell trajectory and transcription factors of embryonic and fetal limb myogenesis.
From: A human embryonic limb cell atlas resolved in space and time
a, Force-directed graph layout of cells associated with the myogenesis, coloured by cell clusters. The green and pink arrows indicate the direction of first and second myogenesis, separately. MyoB, myoblast; MyoC, myocyte; MyoProg, myogenic progenitor. b, Dot plot showing expression pattern of selected marker genes. The colour bar indicates the average expression level in linearly scaled values. c, Fraction of cell type per timepoint. d, Heatmap illustrating the vertically normalized mean activity of filtered genes encoding transcription factors for each cell cluster. e, Violin plot showing the expression level of PITX1 in human forelimb and hindlimb at PCW5.6. f, Immunofluorescence co-staining (scale bar, 50 μm) of PITX1 and PAX3 on hindlimb (top panels) and forelimb (bottom panels) sections (scale bar, 200 μm). Hindlimb, n = 4; forelimb, n = 2. g, RT–qPCR analysis of the fold-enrichment myocyte genes upon knockdown of MSC in human primary embryonic myoblasts. Data are presented as mean ± s.e.m. P values are from two-sided Student’s t-tests. n = 2 embryos and 3 independent experiments with similar results.
