Extended Data Fig. 19: Activity- and size-related signatures of rubrospinal, cerebellospinal, and select reticulospinal neurons.
From: A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons
a, UMAP representation of all SPNs with RuSNs and CbSNs highlighted. b, Expression of RuSN and CbSN marker genes (Rreb1, Spp1) and activity-related genes (Pvalb, Kcng4). c, Volcano plot of differentially expressed genes between Spp1+ and Spp1- RuSNs (SSv4 dataset, N = 1,031 nuclei). Select genes relevant for cell size (Spp1, Nefh, Nefm, Nefl, S100b) and activity (Pvalb, Kcng4, Kcnip4, Hpca, Kcnn3, Kcnc4, Gabrb1) are annotated. Differential expression was performed with Seurat using the “MAST” test; significant genes were defined as those with an FDR adjusted p-value of less than 0.05.
